Class: Extraction/Preservation (148)

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Method for purifying pluri-differentiated mesenchymal progenitor cells

Patent Number: 7,303,769

This patent covers the extraction/purification of a type of mesenchymal stem cell. Specifically the invention claims removing macrophages from a Dexter culture, fractionating cells, and collecting from the factionated cells a stem cell population. The fractionation procedure may be performed by percoll gradient (a type of density gradient). The stem cells that are purified are called...

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Antibody compositions for preparing enriched mesenchymal progenitor preparations

Patent Number: 6645727

This patent covers the antibody kit that can be used for negatively depleting non-mesenchymal stem cell populations from a population of cells in order to obtain a substantially purified population of mesenchymal stem cells.

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Compositions for identification and isolation of stem cells

Patent Number: 7,223,549

This patent covers several antibodies that specifically bind ectodermally-derived stem cell but have weaker affinity for stem cells of mesodermal and endodermal origin. The antibodies seem to bind prostate and liver stem cells but not dermal stem cells. The antibodies are deposited with ATTC and are commercially available. Unfortunately, the ligand for the...

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Making neural cells for human therapy or drug screening from human embryonic stem cells

Patent Number: 6833269

Despite all the publicity of embryonic stem cell therapy, the fact that embryonic stem cells turn into cancer cells when inserted into a living organism suggests the very import need to understand and develop ways of manipulating them to differentiate into the tissue that is needed to be repaired. This patent teaches conditions for generating neural cells from embryonic stem cells through...

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Method for the isolation of stem cells by immuno-labeling with HLA/MHC gene product marker

Patent Number: 6828145

Classically hematopoietic stem cells are purified based on expression of markers such CD34 or c-kit. This patent teaches that hematopoietic stem cells can also be further purified by selecting cells that lack expression of MHC I. The lack of expression of class I may have to do with the presumed weaker immunogenecity of hematopoietic stem...

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Human embryonic stem cells derived from frozen-thawed embryo

Patent Number: 6921632

This patent covers a new way of extracting embryonic stem cells from human blastocyst embryos that have been frozed. The patent is assigned to a Korean Infertility Clinic. It appears that some of the ES cell lines made by this clinic are on the NIH list. The patent has 2 independent claims: A process for making undifferentiated human embryonic stem cells...

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Method for cloning animals with targetted genetic alterations by transfer of long-term cultured male or female somatic cell nuclei, comprising artificially-induced genetic alterations, to enucleated recipient cells

Patent Number: 7071372

Although numerous technologies exist for cloning of animals, it appears from the current patent, that one of the limitations is that the donor cell usually has to be freshly isolated, or cultured for a short period of time. This does not allow for manipulation of the donor cell at a genetic level before nuclear transfer. The current patent teaches methods of long-term culture of donor cells...

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Methods to prepare and use epidermal stem cells

Patent Number: 6927060

This is a method of isolating stem cells by dye exclusion and applying the principle to epidermal stem cells. It is well known in the art that stem cells are the property to exclude various dyes. This is what causes the existance of the "side population cells" which were known since 1996 (Goodell MA, Brose K, Paradis G, Conner AS,Inventors: Bickenbach, Jackie R. (Iowa City, IA); Dunnwald, Martine (Iowa City, IA)