Class: Extraction/Preservation (148)
Markers for identification and isolation of pancreatic islet .alpha. and .beta. progenitors
Patent Number: 6753153This patent discloses protein markers that can be used for purification of pancreatic stem cells. The markers are agents that are capable of binding the protein ErbB2. This patent may be used for purification and ex vivo expantion of islets from tissue, or may be used for characterization of islet stem cells generated via embryonic or other type of stem cell culture.
Method of isolating adult mammalian CNS-derived progenitor stem cells using density gradient centrifugation
Patent Number: 6767738This patent teaches ways of using gradient centrifugation (separation based on weight) to purify stem cells of the neural lineage from adults. This is specifically useful since it is a relatively easy way to purify cells and is commonly used in the purification of hematopoietic stem cells.
Method of providing cells and other biologic materials for transplantation
Patent Number: 6793677This patent teaches ways of using cells and stem cells from the nucleus pulposus for the treatment of degenerative back diseases. Since lower back pain is a major medical concern, various stem cell therapeutics are being considered for stimulation of the nucleus pulposus cellular content, so as to regenerate degenerate discs. The cells used in this patent do not necessarily have to be...
Method of producing an undifferentiated avian cell culture using avian primordial germ cells
Patent Number: 6333192This patent teaches novel ways of generating chicken embryonic stem cells. This patent is useful for veterinary applications, as well, it may be transferrable to other species.
Process for reconstructing a non-human mammalian embryo by nuclear transfer using a heat treated donor nucleus and preparaing a non-human mammal
Patent Number: 7064245One of the issues associated with cloning of animals is that the donor nucleus has to be reprogrammed by the recipient oocyte. Reprogramming involves unraveling of the densely packed DNA chromatin structures which is performed by the oocyte cytoplasmic machinery. In this invention, heat treatment of donor cell or constituents thereof us used to increase the rate of...
Lineage specific cells and progenitor cells
Patent Number: 6929948This patent teaches ways of extracting cells with a neural phenotype from a population of stem cells that are differentiated along the neural lineage through selecting cells that express a marker under control of a promoter which is active only in neural cells. Specifically, the patent claims the use the Sox gene promoter. One way to use this patent would be to insert GFP... When growing neural stem cell cultures, at a certain point in culture they need to be passaged. According to the background in the patent, before the invention, scientists would dissociate neurospheres by mechanical means, which as one could expect causes a loss in cell viability. This patent covers the use of collagenase in dissociating neurospheres when passaging them. This patent is... This patent teaches that tissue samples, or neurospheres, may be dissociated with collagenase instead of mechanical means, in order to maintain viability of the stem cell component. StemCells Inc is one of the few companies that has actually entered clinical trials. In this patent the extraction of specific neuronal stem cells is disclosed. Cells that bind to the antibodies 5E12 and/or AC133 derived from brain sources are identified as neuronal stem cells capable of inducing production of neurospheres. These cells have also demonstrated ability... This patent covers a medical device that is useful for the concentration of stem cells, especially hematopoietic stem cells, from peripheral blood and other fluids.
Use of collagenase in the preparation of neural stem cell cultures
Patent Number: 7049141
Use of collagenase in the preparation of neural stem cell cultures
Patent Number: 6238922
Enriched central nervous system stem cell and progenitor cell populations, and methods for identifying, isolating and enriching for such populations
Patent Number: 6468794
Blood separation system particularly for concentrating hematopoietic stem cells
Patent Number: 6733433