Patents
Pluripotential embryonic stem cells and methods of making same
Patent Number: 5453357This is one of the early embryonic stem cell patents. In this one embryonic stem cells are maintained in LIF, stem cell factor, and fibroblast growth factor. It is interesting to analyze this patent in light of the WARF patents because there is some very interesting similarities to the approaches.
Apparatus and method for culturing embryonic stem cells
Patent Number: 5449620Generation of knockout animals, as well as other chimeras, involves insertion of a gene-targeted embryonic stem cell into an embryo. This patent discloses a device that can physically hold the embryo so that injection of the stem cell can be performed with increased ease and accuracy.
Three-dimensional cell and tissue culture system
Patent Number: 5443950This patent claims a three dimensional structure that can be grown in vitro using stromal cells, stem cells, and other cell types, for the generation of tissue. The structure can then be introduced in vivo either to function as an organ, or to assist the function of an existing body component.
Method for the ex vivo replication of stem cells, for the optimization of hematopoietic progenitor cell cultures, and for increasing the metabolism, GM-CSF secretion and/or IL-6 secretion of human stromal cells
Patent Number: 5437994This patent teaches methods of expanding human hematopoietic cells ex vivo using various cytokine cocktails. The patent is heavily cited by other patents and its claims appear to be far-reaching.
Method of enhancing the growth of gut epithelial cells by administration of a cytokine such as interleukin II
Patent Number: 5437863Gut epithelial cells are proliferating, well at least the stem cells that give rise to them, are in a state of constant proliferation. This is one of the reasons why after chemotherapy and/or radiation therapy, there is adverse effects associated with the GI tract. This patent teaches that proliferation of these cells can be stimulated by IL-6, IL-11, LIF, Oncostatin...
Method for culturing human hematopoietic stem cells in vitro
Patent Number: 5436151This patent teaches how to culture hematopoietic stem cells so as to expand the hematopoietic repopulating ability through the culture on top of stromal cells, however under conditions so that the stromal cells are not in physical contact with the hematopoietic stem cells. There is great interest in methods of culturing and expanding stem cell populations using various types of "feeder...
Proliferative action of leukaemia inhibitory factor on satellite cells
Patent Number: 5435999Satellite cells are muscle stem cells. This patent teaches that LIF either alone, or together with TGF-beta, EGF, or FGF, is capable of stimulating proliferation of muscle satellite cells. This technology is useful not only for stimulating proliferation of endogenous satellite cells after...
Methods and compositions for isolation and growth of kidney tubule stem cells, in vitro kidney tubulogenesis and ex vivo construction of renal tubules
Patent Number: 5429938This patent covers the isolation, expansion, and use of kidney stem cells in order to generate in vitro renal tubules. The compounds used for kidney stem cell expansion include retinoic acid, TGF, and EGF.
Increase in hematopoietic progenitor cells in peripheral blood by transforming growth factor beta
Patent Number: 5426098This patent leverages the ability of TGF-beta to protect stem cells from cytotoxic agents by inhibiting proliferation of stem cells. Specifically, since chemotherapy kills proliferating cells, if the hematopoietic stem cells are first stopped from proliferating by administration of TGF-beta, then the hematopoietic system should be...
System and method for transplantation of cells
Patent Number: 5423778This patent teaches the expansion of stem cells and progeny of the stem cells in vivo in a patient. The patent discloses various chambers, as well as methods of introducing the cells into a patient so that the cells can be truly cultured "in vivo".