CD34 to iPS: A New Look at Banking?

Monday March 30th, 2009 @ 05:11:20 EST

From Category: Hematopoietic
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Boston, MA -

The generation of inducible pluripotent stem cells (iPS) has allowed creation of embryonic-like cells that appear to have similar degree of pluripotency and gene expression.  Originally iPS cells were made from skin cells.  A more recent paper (Loh et al. Generation of induced pluripotent stem cells from human blood. Blood 2009 Mar 18) described the use of adult peripheral blood CD34 cells as a starting population for generation of iPS cells. 

The investigators used mobilization of healthy adults to collect CD34 cells, which were then retrovirally transfected with OCT4, SOX2, KLF4 and MYC.  They found that the artificial iPS cells were identical to embryonic stem cells when assessing:
a) morphology,
b) expression of surface antigens and pluripotency-associated transcription factors,
c) DNA methylation status at pluripotent cell-specific genes, 
d) capacity to differentiate in vitro and in teratomas

So now the question comes...how long will it be before a service is set up for people to make and bank their own "individual specific" iPS cells?


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