Methods and compositions for tissue regeneration

Patent Number: 7,144,729

This patent covers a novel method of treating wounds through "spraying" allogeneic cells, together with fibrinogen and thrombin, in order to secrete various growth factors, including factors that stimulate stem cells, so as to accelerate wound healing.

Systems for inducing tissue regeneration, as well as kits are provided. Basically, the independent claim covers a two part administration of a) a spray of fibrinogen, and b) a spray containing thrombin and cells (allogeneic fibroblasts or keratinocytes) that secrete cytokines. The cells are mitotically inactivated. Cytokines produced by the cells include GM-CSF, VEGF, KGF, bFGF, TGF (alpha and beta), angiopoietin, EGF, IL-I.beta., IL-6, IL-8, and TNF.

This invention is useful for a wide variety of applications such as diabetic wounds, military purposes, and pre-treatment before application of allogeneic or autologous skin grafts.

View this patent on the USPTO website.