Method of culturing cells to produce a tissue sheet

Patent Number: 7,166,464

The ability to generate monolayers of cells is important for a variety of tissue engineering applications. Most notably is the need to generate monolayers that can be placed on medical devices such as stents in order to allow increased compatibility and decreased rates of restenosis.

The current patent teaches ways of culturing cells, including stem cells, in a monolayer and methods of extracting the cells from the monolayer so that they may be used for a variety of purposes.

An important point about the tissue culture conditions described in this patent is that they do not contain xenogeneic proteins and are easily translatable into the clinic.

View this patent on the USPTO website.