Genome mutation by intron insertion into an embryonic stem cell genome

Patent Number: 7,205,148

Genetic manipulation of ES cells provides a powerful method for developing unique cell line models of various human diseases. More clinically applicable is the possibility of specifically producing mutations in genes that elicit rejection of ES cells, so as to generate "universal donor" stem cells.

This patent teaches ways of generating mutations in exons of genes in ES cells by introduction of intronic sequences.

Insertion of intronic sequences is provided by the use of targeted homologous recombination.

View this patent on the USPTO website.