Protocols for making hepatocytes from embryonic stem cells
Patent Number: 7,473,555
Date of First Priority Issue: Thursday April 27th, 2000
Date Issued: Tuesday January 6th, 2009
Assignee: Geron Corporation (Menlo Park, CA)
Inventors: Mandalam; Ramkumar (Union City, CA), Faouzi; Saadia (Daly City, CA), Nadeau; Isabelle (San Francisco, CA), Pfendler-Bonham; Kristina (South San Francisco, CA), Rao; Namitha (San Jose, CA), Carpenter; Melissa K. (London, CA), Rambhatla; Lakshmi (Redwood Ci
From Class: Differentiation
Comments: No comments
This patent covers the generation of mature hepatocytes from pluripotent stem cells (pluripotent I presume they mean to include iPS). The inventors cover a three step process by which they induce pluripotent stem cells to differentiate into endoderm cells, then induce endoderm to differentiate into hepatic progenitors, and lastly induces the hepatic progenitors to differentiate into mature hepatocytes. This process is enacted by culturing the pluripotent stem cells with DMSO, fibroblast growth factor 8 (FGF 8), or a bone morphogenic protein (BMP) to make them hepatocyte progenitors, then culturing in the presence of a histone deacetylase inhibitor, a BMP, epidermal growth factor (EGF), a corticosteroid, or Oncostatin M to induce differentiation into a hepatocyte cell, and inducing maturation of the hepatocyte by culture in hepatocyte growth factor (HGF), or with one or more growth factors in combination with a histone deacetylase inhibitor.
View this patent on the USPTO website.
Added to StemCellPatents.com on Monday January 12th, 2009
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