This patent is directed towards the culture of avian embryonic stem cells. The patent has two independent claims. The first is a culture medium for avian embryonic stem cells comprising of FGF-2, IGF-1; at least one cytokine selected from LIF, IL-11, IL-6, CNTF, and oncostatin M (OSM)) and c-kit ligand (either mouse or avian. The second independent claim covers a method of culturing the avian embryonic stem cells, essentially through administering the same compounds, except that structural support such as a scaffold or feeder layer of cells is also claimed.
View this patent on the USPTO website.
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