Unactivated oocytes as cytoplast recipients for nonprimate mammalian and pig nuclear transfer

Patent Number: 7,432,415

This is one of Ian Wilmut's many patents on cloning animals (for example 7,321,076, 7,304,204, 6,525,243) 

This one covers essentially a method of preparing a nonprimate mammalian embryo capable of developing into a live-born nonprimate mammalian animal comprising:

(a) culturing a diploid differentiated nonprimate mammalian nuclear donor cell;

(b) transferring the cultured diploid differentiated nonprimate mammalian nuclear donor cell or nucleus thereof into an unactivated, enucleated metaphase II-arrested nonprimate mammalian oocyte of the same species to reconstruct an embryo; and

(c) activating the reconstructed embryo to establish the nonprimate mammalian embryo.

The dependent claims include various culture media and various donor cells.

View this patent on the USPTO website.