Method of isolating human neuroepithelial precursor cells from human fetal tissue

Patent Number: 6852532

This patent teaches methods of extracting and purifying neuroepithelial precursor cells that can differentiate into a variety of neurological tissue and be useful for regenerative medicine. The patent essentially teaches to deplete cells expressing the markers A2B5, NG2 and eNCAM, so that approximately half of the purified cells are positive for AC133/2.

The cells are extracted at the time that the donor is undergoing neurogenesis...so this involves extraction of fetal tissue.

The patent teaches that fgf and chick embryo extract are useful for maintaining and propagating such neuroepithelial precursor cells.

View this patent on the USPTO website.