Gene transfer into primate embryonic stem cells using VSV-G pseudotyped simian immunodeficiency virus vectors

Patent Number: 7,323,337

This patent covers methods of transfecting embryonic stem cells. Specifically, it claims the use of SIV genetically engineered so that the nucleic acid which is being transfected is packaged into an SIV virion. Specific proteins and strains of SIV are covered that are useful for transfection. Numerous techniques for genetic modification of hematopoietic stem cells has previously been patented, for example 6277369 which covers the use of TGF-beta for increasing transfection efficacy while inserting the Factor IX gene. The current patent is advantageous since it provides a level of transfection that is much higher in comparision to other means.

View this patent on the USPTO website.