This patent covers a cytokine whose modern name is not known from reading the patent. The cytokine is capable of stimulating expansion of CD34+ human hemapoietic stem cells.
This patent teaches methods of culturing and expanding cells, including stem cells, through a unique culture system that uses hollow fiber technology. Since tissue culture is usually performed under static conditions and very derived from the in vivo situation, numerous failures to expand stem cells can be explained. The culture system of this invention seeks to provide a more in vivo like...
This patent covers ways of growing mammary epithelial tissue in vitro. It teaches that such cells can be grown through dissociating stromal cells using enzymez and mechanical means so as to obtain a population of clumps of epithelial cells. The epithelial cells are then grown in a conditioned media that is either conditioned by human fetal intestine epithelial cells or human bladder...
This patent provides bioreactors and specific culture conditions for the in vitro and ex vivo expansion of hematopoietic stem cells. The patent discloses cytokines, oxygen tensions, and nutrients useful for expansion of cells.