Class: Extraction/Preservation (156)
Human embryonic stem cells derived from frozen-thawed embryo
Patent Number: 6921632This patent covers a new way of extracting embryonic stem cells from human blastocyst embryos that have been frozed. The patent is assigned to a Korean Infertility Clinic. It appears that some of the ES cell lines made by this clinic are on the NIH list. The patent has 2 independent claims: A process for making undifferentiated human embryonic stem cells...
Method for cloning animals with targetted genetic alterations by transfer of long-term cultured male or female somatic cell nuclei, comprising artificially-induced genetic alterations, to enucleated recipient cells
Patent Number: 7071372Although numerous technologies exist for cloning of animals, it appears from the current patent, that one of the limitations is that the donor cell usually has to be freshly isolated, or cultured for a short period of time. This does not allow for manipulation of the donor cell at a genetic level before nuclear transfer. The current patent teaches methods of long-term culture of donor cells...
Methods to prepare and use epidermal stem cells
Patent Number: 6927060This is a method of isolating stem cells by dye exclusion and applying the principle to epidermal stem cells. It is well known in the art that stem cells are the property to exclude various dyes. This is what causes the existance of the "side population cells" which were known since 1996 (Goodell MA, Brose K, Paradis G, Conner AS,Inventors: Bickenbach, Jackie R. (Iowa City, IA); Dunnwald, Martine (Iowa City, IA)
Cultivation of human embryonic stem cells in the absence of feeder cells or without conditioned medium
Patent Number: 7,439,064There are problems with scalability of embryonic stem cells, as well as the fact that animal derived products are conventionally used during their culture. While fetal calf serum has previously been used, and is continued to be used in a lot of the stem cell clinical trials going on, there is always a desire to have "animal free" cells for clinical development. Companies...
Normothermic, hypothermic and cryopreservation maintenance and storage of cells, tissues and organs in gel-based media
Patent Number: 6632666This patent provides novel cellular and tissue storage compositions that are useful for not only stem cells but other cellular therapies.
Enriched preparation of human fetal multipotential neural stem cells
Patent Number: 7,468,277This patent essentially covers a composition of purified human fetal multipotent neural stem cells that are isolated extracted directly from the brain of a human fetus without initial epidermal growth factor expansion. Specifically, the wherein the enriched preparation is capable of: (1) generating neurons, astrocytes, and oligodendrocytes and (2) being propagated for at least 7...
Identification of cells for transplantation
Patent Number: 6465215This patent covers genes that are indicative if cells of the neuronal lineage are suitable for transplantation for regenerative purposes. The genes mentioned in this patent are not named, but sequences are provided. So if one is curious what these are, they have to check genbank.
Markers for identification and isolation of pancreatic islet .alpha. and .beta. progenitors
Patent Number: 6753153This patent discloses protein markers that can be used for purification of pancreatic stem cells. The markers are agents that are capable of binding the protein ErbB2. This patent may be used for purification and ex vivo expantion of islets from tissue, or may be used for characterization of islet stem cells generated via embryonic or other type of stem cell culture.
Method for preparing cell fraction containing hemangioblasts
Patent Number: 7,455,962Therapeutic angiogenesis offers the possibility of treating a variety of diseases associated with ischemia. For example, autologous bone marrow stem cells were used at Indiana University for the treatment of critical limb ischemia patients. Although several studies have used various bone marrow and bone marrow derived...
Method of isolating adult mammalian CNS-derived progenitor stem cells using density gradient centrifugation
Patent Number: 6767738This patent teaches ways of using gradient centrifugation (separation based on weight) to purify stem cells of the neural lineage from adults. This is specifically useful since it is a relatively easy way to purify cells and is commonly used in the purification of hematopoietic stem cells.